Genetics & Molecular Biology

qPCR

Quantitative PCR or real-time PCR is a modification of the standard PCR that allows us to monitor the amount of the amplified product after each cycle in real time.

RT-PCR

RT-PCR is a modification of standard PCR, which uses reverse transcriptase to transcribe RNA into cDNA, which is then amplified using the standard PCR.

PCR

PCR or polymerase chain reaction a very powerful laboratory technique that is used for copying segments of DNA.

DNA libraries

DNA libraries are collections of cloned DNA fragments. There are two main types of DNA libraries, genomic DNA libraries and complementary DNA (cDNA) libraries.

Cloning Vectors

Cloning vectors are DNA molecules with inserted DNA fragments. They are useful for copying fragments of DNA as they can be introduced into a host cell and replicate.